View: UMGi014-C-2 (Genetically Modified Cell Lines)

General

hPSCreg NameUMGi014-C-2
ProviderUniversity Medical Center Goettingen
LocationStem Cell Unit
Cell TypeiPSC
GradeResearch
Underlying Cell LineUMGi014-C
Is the cell line readily obtainable for third parties? Yes
   > Consent for research use? Yes
   > Consent for clinical use? No
   > Consent for commercial use? No

Editing

Editing StrategySNP introduction
Targeted GeneKRAS
Editing MethodCRISPR | protein | nucleofection

Miscellaneous

Contactlukas.cyganek@gwdg.de

Linked Publications

Generation of a genetically-modified induced pluripotent stem cell line harboring an oncogenic gene variant KRAS p.G12V
Viktoria Busley A et al., 2023
 

Clones

Genomic ModificationZygosityHDR/NHEJSpecificationCulture MediumCharacterization
SNP introducedheterozygousHDRKRAS p.G12VStemMACS™ iPS-Brew
SNP introducedheterozygousHDRKRAS p.G12VStemMACS™ iPS-Brew
SNP introducedcompound heterozygousHDRKRAS p.G12V, KOStemMACS™ iPS-Brew
SNP introducedheterozygousHDRKRAS p.G12VStemMACS™ iPS-Brew
SNP introducedcompound heterozygousHDRKRAS- p.G12V, KOStemMACS™ iPS-Brew
KOheterozygousHDRKRAS KOStemMACS™ iPS-Brew